characterization of β-lactamases from urinary isolates of escherichia coli in tehran
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abstract
knowledge of antimicrobial resistance patterns in e. coli, the predominant pathogen associated with urinary tract infections (uti) is important as a guide in selecting empirical antimicrobial therapy. methods: to describe the antimicrobial susceptibility of e. coli associated with uti in a major university hospital in tehran (iran), seventy-six clinical isolates of e. coli were studied for susceptibility to b-lactam antibiotics by the disc diffusion method and minimal inhibitory concentrations determination. results: all isolates were resistant to ampicillin, amoxicillin and oxacillin. resistance to the other tested antibiotics was shown to be 93.4% to cefradine, 76.3% to carbenicillin, 47.3% to cefazoline, 50% to cefalexin and 32.8% to cephalothin while 1.3% expressed resistance to cefoxitime, and 2.6% were resistant to ceftizoxime and ceftriaxone. two isolates (2.4%) harbored extended spectrum b-lactamases (esbl) shown by the double disc diffusion method. substrate hydrolysis by ultra violet spectroscopy showed that 87.4% harbored penicillinases, 9% produced cephlosporinases and 3.6% degraded both substrates. clavulanic acid inhibited enzyme activity in 82.9%, of which 78.95% was penicillinases (group iia) and 3.95% was cephalosporinases (group iib) of the bush classification system. the rest of the isolates (6.58 %) were placed in group iv b-lactamases. no group iii b-lactamase was found, as edta inhibited none of the enzymes. dna amplification by polymerase chain reaction using specific primers for ampc, tem and shv type b-lactamases for all of the isolates showed that 47 organisms (60%) carried the tem gene and 18 isolates (24%) harbored blatem and ampc genes. about 26% of the organisms harbored shv type enzymes. conclusion: these results indicate that e. coli can posses a variety of b-lactamases that are responsible for β-lactam resistance.
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Journal title:
iranian biomedical journalجلد ۱۱، شماره ۲، صفحات ۹۵-۹۹
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